ACRIDINE ORANGE For Microscopical Staining

Short Name: ACRIDINE ORANGE

Other Names: Basic Orange 14, Euchrysine, N,N,N',N'-Tetramethylacridine-3,6-diamine

C.I.No.46005Riboneucleic acid stain -Acridine orange (AO) is a nucleic acid selective fluorescent cationic dye useful for cell cycle determination. It is cell-permeable, and interacts with DNA and RNA by intercalation or electrostatic attractions.It is also useful as a non-specific stain for backlighting conventionally stained cells on the surface of a solid sample of tissue (fluorescence backlighted staining)

Chemical Specifications

Appearance : Dark brown colored powder

Absorption maxima (50% ethanol) : 489 - 493 nm

Absorptivity (A 1%/1cm; at λmax; 50% ethanol) : Min 950

Loss on drying (at 110°C) : Max 5%